{"id":4623,"date":"2024-05-20T05:47:00","date_gmt":"2024-05-20T05:47:00","guid":{"rendered":"https:\/\/blog.ki.se\/studentbloggen\/?p=4623"},"modified":"2024-05-19T17:40:52","modified_gmt":"2024-05-19T17:40:52","slug":"praktik-pa-bakteriologen","status":"publish","type":"post","link":"https:\/\/blog.ki.se\/studentbloggen\/2024\/05\/20\/praktik-pa-bakteriologen\/","title":{"rendered":"Praktik p\u00e5 Bakteriologen"},"content":{"rendered":"

F\u00f6rra m\u00e5naden var jag ute p\u00e5 min f\u00f6rsta verksamhetsf\u00f6rlagda utbildning, allts\u00e5 min praktik. Det var j\u00e4ttekul och jag har l\u00e4nge sett fram emot att f\u00e5 komma ut p\u00e5 praktik och se n\u00e5gra sp\u00e4nnande laboratorier! Jag blev placerad p\u00e5 Bakteriologen i Huddinge och jag fick l\u00e4ra mig en massor.\u00a0<\/span><\/p>\n

V\u00e5r praktik var totalt sju dagar, och jag fick f\u00f6lja med p\u00e5 tv\u00e5 avdelningar p\u00e5 patologen. Den f\u00f6rsta avdelningen jag var p\u00e5 kallas f\u00f6r\u00a0Luft<\/em>. D\u00e4r analyseras olika bakterieprov som \u00e4r relaterade till sjukdomar i luftv\u00e4garna, till exempel prover som tagits fr\u00e5n n\u00e4sa och svalg. Efter det fick jag \u00e4ven h\u00e4nga med p\u00e5 en avdelning d\u00e4r de bland annat hanterade bakterieprover fr\u00e5n bland annat proteser och olika v\u00e4vnader!<\/p>\n

Hur fungerar analys av bakterier?<\/h3>\n

D\u00e5 jag inte f\u00e5r ber\u00e4tta exakt hur metoderna s\u00e5g ut p\u00e5 kliniken, t\u00e4nkte jag ist\u00e4llet sammanfatta hur man generellt brukar g\u00f6ra f\u00f6r att analysera bakterieprovet – s\u00e5som vi har l\u00e4rt oss!<\/p>\n

F\u00f6r att kunna analysera f\u00f6rekomsten av bakterier m\u00e5ste man f\u00f6rst g\u00f6ra n\u00e5gon sorts utodling\u00a0<\/em>av provmaterialet. Bakterier \u00e4r mycket sm\u00e5 och \u00e4r ofta inte i tillr\u00e4cklig stor m\u00e4ngd, eller utblandat med annat material fr\u00e5n d\u00e4r provet tagits, f\u00f6r att kunna analyseras direkt. Av den anledningen m\u00e5ste man d\u00e4rmed f\u00f6rst odla ut bakterierna och l\u00e5ta dem v\u00e4xa till sig f\u00f6rst. Allra vanligast odlar man ut provet p\u00e5 en s\u00e5 kallad agarplatta<\/em>, som \u00e4r ett sorts gel\u00e9liknande \u00e4mne som inneh\u00e5ller n\u00e4ring till bakterierna. Det finns massor av olika sorters agarplattor som anv\u00e4nds i olika syften. Till exempel finns det speciella agarplattor d\u00e4r endast specifika bakterier faktiskt kan v\u00e4xa. Dessa agarplattor beh\u00f6ver sedan inkuberas <\/em>i olika milj\u00f6f\u00f6rh\u00e5llanden (temperatur, syre\/koldioxid) f\u00f6r att l\u00e5ta bakterierna v\u00e4xa. N\u00e4r bakterierna sedan har vuxit (eller inte) s\u00e5 b\u00f6rjar sj\u00e4lva analysen.<\/p>\n

\"En

En ritning p\u00e5 hur en agarplatta med bakteriev\u00e4xt kan se ut. K\u00e4lla: Originalritning av mig (Maja)<\/p><\/div>\n

Det finns en massor av olika analyser som g\u00e5r att g\u00f6ra f\u00f6r att identifiera bakterier, men f\u00f6r en riktigt erfaren biomedicinsk analytiker g\u00e5r det ibland att se direkt p\u00e5 det makroskopiska utseendet vilken bakterie det \u00e4r! Det var v\u00e4ldigt h\u00e4ftigt att se n\u00e4r mina handledare visade mig en platta och direkt kunde s\u00e4ga vad det var baserat p\u00e5 hur de sm\u00e5 bakteriekolonierna s\u00e5g ut! F\u00f6r att vara helt s\u00e4ker m\u00e5ste dock n\u00e5gon sorts verifierad analys anv\u00e4ndas f\u00f6r att bekr\u00e4fta vilken\/vilka bakterier som vuxit fram. Ett exempel \u00e4r agglutinationstest som kan g\u00f6ras f\u00f6r specifika bakterier. D\u00e5 blandar man en liten bit av bakterien med provreagenser, och om det \u00e4r just den specifika bakterien, kommer l\u00f6sningen att f\u00e5 sm\u00e5 klumpar (agglutinat).<\/p>\n

MALDI-ToF – laser och flygtid!<\/h3>\n

Den allra vanligaste analysen f\u00f6r bakterietypning (och ofta den mest f\u00f6redragna) heter MALDI-ToF. MALDI-ToF \u00e4r en f\u00f6rkortning av Matrix-Assisted Laser-Desorption Ionization-Time of Flight. Det l\u00e5ter ju j\u00e4ttekomplicerat, och likt alla analysmetoder f\u00e5r det mig alltid att t\u00e4nka:\u00a0hur lyckades n\u00e5gon komma p\u00e5 det h\u00e4r?\u00a0<\/em>S\u00e5 nu ska jag f\u00f6rs\u00f6ka f\u00f6rklara analysen med lite enklare termer!:<\/p>\n

MALDI-delen av analysen \u00e4r en joniseringsteknik, vilket inneb\u00e4r att man vill ge analysmaterialet n\u00e5gon sorts laddning. Det h\u00e4r g\u00f6r man genom att l\u00e4gga bakterierna tillsammans med ett sorts ljusabsorberande material (matrix). Vid detta steg d\u00f6das \u00e4ven bakterierna s\u00e5 att deras inneh\u00e5ll, med bland annat en massor av proteiner, l\u00e4cker ut! Sedan s\u00e4tter man in provet i en maskin med en laser som skjuter mot provet. Laserns ljus absorberas sedan av detta matrix s\u00e5 att den f\u00e5r massor av energi. Den h\u00e4r energin anv\u00e4nds sedan f\u00f6r att g\u00f6ra proteinerna fr\u00e5n bakterierna till joner – allts\u00e5 f\u00e5r de en laddning.<\/p>\n

Sen kommer det roliga – Time of Flight. I Time of Flight-analysatorn tar man proteinerna som nu har blivit laddade, och kastar upp dem i en kolumn – och m\u00e4ter tiden det tar f\u00f6r dem att n\u00e5 till m\u00e5llinjen! Enligt principen kommer proteiner av olika storlekar d\u00e5 att ta olika l\u00e5ng tid p\u00e5 sig att n\u00e5 m\u00e5llinjen, och i slut\u00e4ndan f\u00e5r vi som en graf \u00f6ver hur mycket av olika proteiner som hittats under analysen. Det h\u00e4r kallas f\u00f6r ett masspektrum! Dessa spektrum \u00e4r specifika f\u00f6r olika bakterietyper, eftersom olika bakterier har olika proteiner i olika m\u00e4ngder. De spektra som man f\u00e5r ut vid analysen j\u00e4mf\u00f6rs sedan med olika databaser f\u00f6r att f\u00f6rs\u00f6ka hitta vilken bakterie som spektrumet passar b\u00e4st till. Och vips, s\u00e5 har vi (f\u00f6rhoppningsvis) f\u00e5tt en tr\u00e4ff p\u00e5 vilken bakterie vi hade!<\/p>\n

Det k\u00e4nns lite som magi ibland, men det \u00e4r bara lite avancerad kemi! S\u00e5dana h\u00e4r metoder och m\u00e5nga fler l\u00e4r vi oss om p\u00e5 biomedicinska analytikerprogrammet. Om du \u00e4r mer intresserad av att f\u00e5 h\u00f6ra om lite olika analyser, och hur det ser ut bakom kulisserna\u00a0<\/em>p\u00e5 olika laboratorier – h\u00e5ll utkik h\u00e4r p\u00e5 bloggen!<\/p>\n

\/Maja, biomedicinsk analytikerstudent<\/p>\n","protected":false},"excerpt":{"rendered":"

F\u00f6rra m\u00e5naden var jag ute p\u00e5 min f\u00f6rsta verksamhetsf\u00f6rlagda utbildning, allts\u00e5 min praktik. Det var j\u00e4ttekul och jag har l\u00e4nge sett fram emot att f\u00e5 komma ut p\u00e5 praktik och […]<\/p>\n","protected":false},"author":594,"featured_media":4627,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[123,36],"tags":[118,121,94,119,25,12,43],"class_list":["post-4623","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-biomedicinska-analytikerprogrammet","category-praktik","tag-biomedicinsk-analytiker","tag-biomedicinska-analytikerprogrammet","tag-bma","tag-bma-programmet","tag-karolinska-institutet","tag-ki","tag-vfu"],"acf":[],"yoast_head":"\nPraktik p\u00e5 Bakteriologen - Studentbloggen<\/title>\n<meta name=\"description\" content=\"F\u00f6rra m\u00e5naden var jag ute p\u00e5 min f\u00f6rsta verksamhetsf\u00f6rlagda utbildning, allts\u00e5 p\u00e5 praktik, p\u00e5 bakteriologen!\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"http:\/\/blog.ki.se\/studentbloggen\/2024\/05\/20\/praktik-pa-bakteriologen\/\" \/>\n<meta property=\"og:locale\" content=\"sv_SE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Praktik p\u00e5 Bakteriologen - 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